Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFDFNLILVPVTLILFAVWLLDKLVFKQRANKGRENENFVITWAYDFWPVLAVVLVLRSFTYEPFNIPSDSMVPTLETGDFILVNKFDYGVRLPIVNKKVIDVGEPKRGDVIVFRYPPQPTISYIKRVIGLPGDHIVYDHGQLIINGQKIPKVPTQFSREKDALDTPTSIYHKETIGDHTFTMRELEGVNVARQAPFINYVDNGKYANQDGLYWEVTVPKGHYFAMGDNRDQSADSRFWGFVPEENLTGRAFYVWMHKEPGFHLPSFNRNGKID 4WVI Chain:A ((377-530)) -------------------------------------------------------------IVTPYTIKGESMDPTLKDGERVAVNIVGYK------------TG-LEKGNVVVFHANK--NDDYVKRVIGVPGDKVEYKNDTLYVNGKKQDEPYLNYNLKH--KQG---DYITG-----TFQ-----V--------------KDL---PNANPKSNVIPKGKYLVLGDNREVSKDSRAFGLIDEDQIVGKVSFRFWSHPQ--------------

General information: TITO was launched using: RESULT: Template: 4WVI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 710 -33316 -46.92 -217.75 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -46.92 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_4WVI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WVI-query.scw PDB file : Tito_Scwrl_4WVI.pdb: