Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKKQIITIAGGGST-YTPGIVQAILNNEKRL--PLSEIRLYDIDEERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRV-------GGLEMREKDEKIPLKHGLV----GQETCGLGGFAYGMRS---IKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMPEIIEKLITKEMQVADFN-IGDKTWQKTFQMMSVITKNFPSNIPN------NYLEYYLYPDMVVEHTD--------KEYTRANMVMDGRE---KNTKEMADKIRRGI---VEEVLNF--NFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISD-FHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK 3U95 Chain:F ((1-458)) -----MKISIVGAGSVRFALQLVEDIAQT-DELSREDTHIYLMDVHERRLNASYILARKYVEELNS-PVKVVKTESLDEAIEGADFIINTAYPYDPRYHDSGSQRWDEVTKVGEKHGYYRGIDSQELNMVSTYTYVLSSYPDVKLALEIAEKMKKMAPKAYLMQTANPVFEITQAVRR-WTGANIIGFCHGVAGVY-EVFERLGLDPEEVDWQVAGVNHGIWLNRFRY--RGKDAYPLLDEWIEKELSKWEPKNPWD-TQMSPA---AMDMYRFYG-MLPIGDTVRNG--TWKYHYNLETKKKWFRRFGGIDNEVERPKFHEHLRRARERLIKLAEEVQENPHLKITEKHPEIFPKGRLSGEQHIPFINAIANNKRVRLFLNVENQGALKDFPDDLVMELPVWVDSSGIHREKVEPDLTHRIKIFYLWPRILRTEWNLEAFISRDRKVLEEILIRDPRTKSYEQVVKVLDEILSLP--------

General information: TITO was launched using: RESULT: Template: 3U95.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 2308 53430 23.15 128.13 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain F : 0.70 3D Compatibility (PKB) : 23.15 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_3U95.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U95-query.scw PDB file : Tito_Scwrl_3U95.pdb: