Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKV-SVYQQKKG--------QAGKLHWDEE--DIFLITYGDQFFEEQQPTLRTFKKFYQK--YLADT-FRVVHFLPFFPYT---SDDGFSVVNYQAINDRLGTWEDIEEMRSET-----RLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDTD-------TSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLNPVR------GILPEKTILELVEQLKK-EGARVNYKKNPDGTTSPYEINATYLDA--------LSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEK---TGENRKINRQKFLLADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSAD-SFISFIREAKEEKVLVIHNFSNQSAGYLLPKG-----DYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK 1G5A Chain:A ((44-627)) --MNELDSVYGNNEALLPMLEMLLAQAWQSYSQRNSSLKDIDIARENNPDWILSNKQVGGVCYVDLFA-------GDLKGLKDKIPYFQELGLTYLHLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIF-PDQHPGGFSQL-EDG--RWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVAFIWKQMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSEAIVHPDQVVQYIGQ--DECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDG--SF-----ARGVPFQYNPSTGDCRVSGTAAALVGLAQDDPHAVDRIKLLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQRNDPSTAAGQIYQDLRHMIAVRQSNPRFDG-GRLVTFNTNNKHIIGYIRN---NALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVS--LNQDLTLQPYQVMWLEI--

General information: TITO was launched using: RESULT: Template: 1G5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2959 -11219 -3.79 -21.13 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -3.79 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_1G5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G5A-query.scw PDB file : Tito_Scwrl_1G5A.pdb: