Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEERFDWMTFGDDVSGAQAGSAAPPSTGADEDVPIFLREDVGMVRAGASHATAASESTGVDSENGAPAFLAVCPGADVAPRLSGGWSSEGSPVWDGSLRQQQKPSSTAALGHPGGAGDVQAAPSAALYADQPSAAVLHFTGDSGRSWGALHRVRILGKGSYGCATLYSMKDAASGGVEPTHAVVVKDINMQTMLN----PAEEVLAVQNELKVLRTVLGHPNLVQYVDALFDTRPPTYPMSFIMMEYCAGGDLAAVMDGRRSDSATTNGAPSSPPSIPLAITTASTAAGPDILTEPCVASLFIQVAVALQSLHTEYGILHRDVKPHNIFLLEDGITVRLGDFGISTQLDRVGDTAKEACGSPYYMAPELFE------ERAYGAAADVWSLGVVFYQLIARQLPFTAASAAELRSLVCRGRCTPLHDLQNEAASRYSRQFKELVSSLLTVDAAARPTLRRVLRHKYVRESLKYVPASVLTAKKPLPPARVPSAGAMPSSSLQRFSADGLYAGLFGSDVVEQAVSQAVHAGVYPIVSRVHSA 2Y7J Chain:D ((92-364)) -------------------------------------------------------------------------------------------------------------------------------------------------FYQKYDPKDVIGRGVSSV--VRRCVHRA-----TGHEFAVKIMEV---RLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESS-----SFMFLVFDLMRKGELFDYLTEKV------------------------------ALSEKETRSIMRSLLEAVSFLHAN-NIVHRDLKPENILLDDNM-QIRLSDFGFSCHLEPG-EKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFS----SPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFE-------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1203 -26957 -22.41 -103.68 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -22.41 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_2Y7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y7J-query.scw PDB file : Tito_Scwrl_2Y7J.pdb: