Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQLVPLVELPSGKKIYSVRGQQFEVDRQYDLVKVVGFGACGTVCSAVANGSGERVAIKRLSRVFGDLREGKRILREMEIMTSLKHNNLIRLHHFMRPQSK-ETFEDIYLVMDLYDTDLNRIIRSRQKLTDEHLQYFMIQAFRGLHYLHSAKVMHRDLKPSNLLVNADCALAICDFGLARDDQVMSSSDLTQYVVTRWYRPPEVLGMGSNQYTSAVDVWSLGLIFAELMLGRTLLPGTDYIGQLVMIVNLLGSPSIDDMEF-LSSEAKAFILSQPRRPALSFRDLFPMATEEATDLLSKLLVFHPARRLTAKQVMEHPYFSKYRDAAEEADAPDPFVWNHSH-IETKEQLREDLWRVVEAHSQSNG 2FST Chain:X ((24-354)) ----------------------IWEVPERYQNLSPVG----GSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLN------QKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILD-------------------ATRWYRAPEIML-NWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVAD-PYDQSLESRDLLIDEWKSLTYDEVIS------

General information: TITO was launched using: RESULT: Template: 2FST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1410 -56365 -39.97 -185.41 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain X : 0.76 3D Compatibility (PKB) : -39.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_2FST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FST-query.scw PDB file : Tito_Scwrl_2FST.pdb: