Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence LCTTIQKGQIFRNILTCMSEFYIKEEFSYTVCNGKNYASPNRTILCIWEGHIIHVRLDERC-NWTRLEGDQTEEL-ESQNVE-FKVLQKCVASKYKLDPPHAWDAGHGVKLG 4NOO Chain:B ((28-122)) -CND--TSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDD---FYSKQRLAIHEKCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS-----------

General information: TITO was launched using: RESULT: Template: 4NOO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 222 -14454 -65.11 -157.10 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -65.11 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4NOO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NOO-query.scw PDB file : Tito_Scwrl_4NOO.pdb: