Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PEHCFVRILKYEIPIHGDGYLVGSDVNWWVGNQKVDFHINNDCTIQRRGGTQEKKPYRLDSISTLGEDLPKGFDFILFRESKIDEFLAQGGVTKRILGSMIL 5LUS Chain:A ((19-68)) -------------------------------------------------HMEQQLYAVMDDICKLVDAIP---LHELEMLSCAKELLLQRGLRRKLLANAVD

General information: TITO was launched using: RESULT: Template: 5LUS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 53 -6241 -117.75 -124.81 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -117.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_5LUS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LUS-query.scw PDB file : Tito_Scwrl_5LUS.pdb: