Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAV---QSAHRAATRG--FIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTGSLFRINVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQ-KDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSV-IDKQDKD 5JJO Chain:A ((4-268)) -------LRPFIC--------VNEKDHLPSLDPQADAWYREAVALAKPDTLRP-WDRIVDLYSKAVERGHWKAMHNLASLYRT----GW--KDTQKALDLYQKMIDLKVPQGFYDMAAMIGNRAGVKNPATDGLTFLDKAASLGNPPALTELGRLYIYVA-----GQDELGLKYTNCAAGQGYAPANYELAMYYRL--VAHNYPKAAGYYLLAASQGNDDAAFFMSGVFDKTSPDVDRM--WYAPDEKLHKLYDGIYDQLAAD-PDLRFPNLIKDHPLPPHPTQGYDADRPD

General information: TITO was launched using: RESULT: Template: 5JJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 -9264 -7.66 -36.61 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -7.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_5JJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JJO-query.scw PDB file : Tito_Scwrl_5JJO.pdb: