Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNSKYITCLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVRSSVERVIEMIITENLTECINQPLDDSE-AQKERLEKAIRYLIKRK 4ADZ Chain:A ((50-133)) -QKAEHLKRLRRIEGQIRGLQRMVDEDVYCIDILTQVSASTKALQSFALQLLEEHLRHCVADAALKGGTEIDAKVEEATKAIGRL-

General information: TITO was launched using: RESULT: Template: 4ADZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 112 -11444 -102.17 -137.87 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -102.17 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_4ADZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ADZ-query.scw PDB file : Tito_Scwrl_4ADZ.pdb: