Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQVESSRATYFDHLFTAVSAVCVTGLSTLPVAH---TYNIWGQIICL------LLIQIGGLGLMTFIGVFYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQLGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITG---GLGFMVWFDLAGHV--GRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNA-GTIGNLPVADKVLVSFFQTVT----MRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVFARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIG---LILLGITAKGNPPFIHLVFETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIHYMKADISIG 5UAM Chain:A ((23-462)) -----DRQVGYFADNGVGNPLAIVQHPAGIHKNGITYVSYQGPK-EDPYIASYNHQTGQWQGPFRAGISELGRRDGGKKFDNHGKPTMLIDDEGYIHIFYGGHGGQASNGKNPLGNTHHGA-NKHAVSKRPYDISQWEDLNNITPFGTYNQAIKMDNGDIYLFFRH--------GAHRSDWVYQKSVDN-GRTFASPVSFLKHKRRTDIDAVDSWYAWAGKGQGDNIIVSYDYHVCWDGGAGVNGRGHTTERH----DVYFMSFNTKTG---EWSNVEGEKLVLPVTREVADEKTMAMRTGELWTFNGSTHLD-AQGQPHIAINAGID------KGAKTGGPKQTRHVRW---NGNEWVGGDKVIPQYERVSRGDFMVTDPENIRYLTTYNQDNDAVLSWWQSHDGGEHFVEDKTVLRKDNASFAISAFIKDAIPDAQMLVAEKVSDEG----IKMYLV-GEEGAVTRSLVDLKTAMP---

General information: TITO was launched using: RESULT: Template: 5UAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2723 -87269 -32.05 -208.78 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -32.05 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_5UAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UAM-query.scw PDB file : Tito_Scwrl_5UAM.pdb: