Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFFICNLVRVVQSPRFY-MSLFLTLLCMSLGNFLAFNGIYKIE---GLSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVGSTAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTL---WYGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLA-LGYQKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV 5L2D Chain:A ((19-252)) --------------PMWVDFSDTASMKNLDPQGGFKVGTVFKKEISPGYVVTLTVTELKPFNSTEIYKKRVEGTPTANTYDPNAINSYLKGY--KDYGKTPPSVTGRTQII------LPDDAVNWGIKFKVEATYRGNPVKPSVVMADGEDANPAEYGIFTTNGEGWEYVGEWMKGPYTVMTEDMVKAFDKTRKDGLLILKDKSVDWSK----YLSPDTVTGGLGSQ----------------------VFGPIISAS----

General information: TITO was launched using: RESULT: Template: 5L2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 -21196 -25.82 -104.93 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -25.82 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_5L2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L2D-query.scw PDB file : Tito_Scwrl_5L2D.pdb: