Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVARDAR----------GELVNVLEDKLEKQAYTCPACGG---QLHLRQGPSVR-THFAHKSLK------------DCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNLALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGF---LYFSQNMGFYVWELDKGKQVLRLKYLIYQDLRG---KLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYG--LKEWYPQIRPIVGK--FFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE 2W0C Chain:L ((1-335)) MIVKKKLAAGEFAETFKNGNNITIIKAVGELVLRAYGADGGEGLRTIVRQGVSIKGMNYTSVMLHTEYAQEIEYWVGDLDYSFQEQTTKS-RDVNSFQIPLRDGVRELL--PEDASRNRASIKSPVDIWIGGENMTALNGIVDGGRKFEAGQEFQINTFGSVNYWVSDEEIRVFKEYSARAKYAQNEGRTALEANN---VPFFDIDVPPELDGVPFSLKARVRHKSKGVDGLGDYTSISVK---PAFYITEGDETTDTLIKYTSYGST-----GSHSGYDFDDNTLDVMVTLSAGVHRVFPVETELDYDAVQEVQHDWYDESFTTFIEVYSDD--PLLTVKGYAQILMERT--

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1208 73885 61.16 247.11 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain L : 0.68 3D Compatibility (PKB) : 61.16 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.085

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: