Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIIKEKISSLLS-QE----EEVLSVEQLGGMTNQNYLAKTTN----------KQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTS--IKTKFDKIAPILQTIHTSAKELRGE--F----APFEEIKKYESLIEEQIPY--AN-Y-ESVRNAVFSLEKRLA------DLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEF------------------TSQEEETFLSHYESDQT----P------VSHEKIAIYKILQDTIWSLWTVYKEEQG-----EDFGDYGVNRYQRAVKGLASYGGSDEK 3C5I Chain:D ((18-359)) -----KKICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKYKIAPQLLNTF--NGGRIEEWLYGD-PLRIDDLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTN-KCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRLKMYDDQKEYL------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1057 15572 14.73 57.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : 14.73 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C5I-query.scw PDB file : Tito_Scwrl_3C5I.pdb: