Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKNIYFVVLDLHT-TDRDKIIQLFKDWTDYSAKLVEGELVKKDGQNALFPPSDTGETVGLNPHRLTLTFGVSASFLKRMNLENKRPRLFRDLPLFPKEQLREKYTGGDIVIHACADDEQIAFHAIRNLIRKGRNAVPLRWSQSGF----AAIGDRMETPWNLFGFKDGTANPTK-EQDFDRVIWADSKD--WMENGSYMAVRRIQMFLETWDRTSLEEQENTFGRYKESGAPF---GKKNEFDEVDLS--------LLPDDSHVCLAK---EVDKPLLRRSYSYSDGIDEKTGQFDTGLLFISFQKDP-DNFVKVQTNLGATDKMNEYITHIGSGLFTCFGGVEKGGYIGQKLLEG 5MAP Chain:B ((26-376)) MQARGHLVAFDLAAGAGRKEAAALLRRWSDTARRLMAGEPAGS---------RDTDVARDAGPSSLTVTFGFGHSFFGRTGLEKQRPVALDPLPDFSSDHLDKNRSNGDLWVQIGADDALVAFHALRAIQRDAGAAARVRWQMNGFNRSPGATAHPM-TARNLMGQVDGTRNPKPGEADFDRRIFVPEE-PAWMANGSYVVVRRIRMLLDDWEELSLKAQEDVIGRRKSDGAPLSGGSGATESTEMDLEKTDGSGELVVPINAHARITRPDQNGGAAMVRRPFSYHDGFDAD-GVPDAGLLFVCWQADPLRGFVPVQRKLDRGDALSQFIRHEASGLFAVPGGAAEGEYVGQRLLE-

General information: TITO was launched using: RESULT: Template: 5MAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1603 18788 11.72 58.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 11.72 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_5MAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MAP-query.scw PDB file : Tito_Scwrl_5MAP.pdb: