Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVS--EMTKDKLIEMMSYSSD-CFEFLSPNLLRFKNRD------NLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLEEARALLPRDTKQLIYMAQTHYLSN 2M46 Chain:A ((1-117)) MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGML-VKRPLAVMGDKITLGFKEDQYKETWL-----------------

General information: TITO was launched using: RESULT: Template: 2M46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 430 -6314 -14.68 -58.46 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -14.68 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2M46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M46-query.scw PDB file : Tito_Scwrl_2M46.pdb: