Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------MVMSLITHRRFISCNE-NIKHYKRLIDKAEKCVN-DLMAELNSVITTVTGIGNRLGAVILAEIQNIHAFDNPAQLQAFAGLDSSIYQSGQID-LAGRMIKRGSPHLR----------------------------------------------------------------------------------------------------- 4INO Chain:A ((34-335)) QEVKVKDYFGEQTIKLPVSKIIYLGSFAEVPAMFHTWDRVVGISDYAFKSDIVKATLKDPERIKPMSSDHAAALNVELLKKLSPDLVVTFVGNPKAVEHAKKFGISFLSFQEKTIAEVMEDIDTQAKALEVDASKKLAKMQETLDFIAERLKGV--KKKKGVELFHKANKISGHQALDSDILEKGGIDNFGLKYVKFGRADISVEKIVKENPEIIFIWWISPLSPEDVLNNPKFATIKAIKNKQVYKLPTMDIGGPRAPLISLFIALKAHPEAFKGVDINAIVKDYYKVVFDLNDAEVEPFLWH

General information: TITO was launched using: RESULT: Template: 4INO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 402 9490 23.61 93.04 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 23.61 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.115

(partial model without unconserved sides chains): PDB file : Tito_4INO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4INO-query.scw PDB file : Tito_Scwrl_4INO.pdb: