Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHFTFLGTSSGVPT--------------------LSRNVSGLAVRNSKNK-DWILVDAGEGTQHRIQQAR-------LSLQNLIAICITHVHGDHCYGLVGLLASAGMNARNNPLIVIAPKEIQQWFEITAQLTDLHL--PYSIKFIDVNEATRPQQLT---DELFIQAHPLSHRV-------------PSFAFSIYIKSTQKKIDIQALTQLGIPKGDIWGHLKRGYDVEFEGRILKSQDFIKIQNQQIHAIIGGDN-DRPELLADACKDAQLLIHEATYLQTVLD--------KVGKGPMHSSAKMVA---EFAEQQSLDNLILTHFSPRHQDKTGQQAIIEEVRQFYKGHFYLANDFDEFTLNETGQLLKIE 4Z67 Chain:A ((1-307)) MMYIQVLGSAAGGGFPQWNCNCVNCKGYRDGTLKATARTQSSIALSD--DGVHWILCNASPDIRAQLQAFAPMQPARALRDTGINAIVLLDSQIDHTTGLLSLRE-------GCPHQVWCTDMVHQDLTTGFPLFNMLSHWNGGLQWNRIEL-E-GSFVIDACPNLKFTPFPLRSAAPPYSPHRFDPHPGDNLGLMVEDTR-----------------------------------------------TGGKLFYAPGLGQVDEKLLAMMHGADCLLVDGTLWEDDEMQRRGVGTRTGRE-MGHLAQNGPGGTLEVLDGFPRQRKVLIHINNTNPILD---ENSPERAEVLRRGVEVAFDGMSIELLEH-------

General information: TITO was launched using: RESULT: Template: 4Z67.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1348 -8653 -6.42 -34.75 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -6.42 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4Z67.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z67-query.scw PDB file : Tito_Scwrl_4Z67.pdb: