Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRIETEASAHKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKLTAPIGNLKTYWQLL-KNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDI-DCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPSGHMGVVSGSQAPTSVWPEMSNWLAMRSD 1AZW Chain:A ((1-313)) ------MRTLYPEITPYQQGSLKVDD----------------------------------------RHTLYFEQCGNPHGKPVVMLHGGPGGGC------NDKMRRFHDPAKYRIVLFDQRGSGRSTPHADLVDNTTWDLVADIERLRTHLGVDRWQVFGGSWGSTLALAYAQTHPQQ-VTELVLRGIFLLRRFELEWFYQEGASRLFPDAWEHYLNAIPPVERADLMSAFHRRLTSDDEATRLAAAKAWSVWEGATSFLHVDEDFVTGHEDAHFALAFARIENHYFVN-----------GGFFEVEDQLLRDAHRIADIPGVIVHGRYDVVCPLQSAWDLHKAWPKAQLQISP-ASGH------SAFEPENVDALVRATDGFA-

General information: TITO was launched using: RESULT: Template: 1AZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1816 -78873 -43.43 -253.61 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -43.43 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_1AZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AZW-query.scw PDB file : Tito_Scwrl_1AZW.pdb: