Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCEFISEYSEFQYDFISLAQQRFVSVPEIDLRTMIQNFGD-----WYFANESSSLNTPSIWLVKWFSWVQNNEKQVAANR--KKQEQITSTCQKPQESGYFANLFEEQSESQIVDVTPAKKFPMIEEVGHA 2LU7 Chain:A ((1-82)) -------------------MTRVRSTPGGDLELVVHLSGPGGPVRWYKDGERLASQGR-------VQLEQAGARQVLRVQGARSGDAGEYLCDAPQDSRIFLVSVEEP-----------------------

General information: TITO was launched using: RESULT: Template: 2LU7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 302 32755 108.46 436.73 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 108.46 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.125

(partial model without unconserved sides chains): PDB file : Tito_2LU7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LU7-query.scw PDB file : Tito_Scwrl_2LU7.pdb: