Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSNDNDAYVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 5IVA Chain:B ((3-151)) --------------------NTSGFQLRGLG-DAQ-FALKEIDVSARNAY-GPTVRELKETLENSGVKVTSN-APYHLVLVREDNQQRTVSYTGSARGAEFELTNTINYEIVGANDLVLM-SNQVQVQKVYVHDENNLIGSDQEAAQLRSEMRRDLIQQLSMRLQALTPAQLDE

General information: TITO was launched using: RESULT: Template: 5IVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 496 25141 50.69 174.59 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 50.69 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_5IVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IVA-query.scw PDB file : Tito_Scwrl_5IVA.pdb: