Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAANVIVYSTSVCPYCVRAKQLLERKGVAYKEVNLS--VEAPEVRAELMQRTNHRTVPQIFINDQFIGGFDQLYALEREGKLDELLA 2E7P Chain:C ((19-103)) -SAPVVVFSKTYCGYCNRVKQLLTQVGASYKVVELDELSDGSQLQSALAHWTGRGTVPNVFIGGKQIGGCDTVVEKHQRNELLPLL-

General information: TITO was launched using: RESULT: Template: 2E7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 331 -17988 -54.34 -216.72 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -54.34 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.821

(partial model without unconserved sides chains): PDB file : Tito_2E7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E7P-query.scw PDB file : Tito_Scwrl_2E7P.pdb: