Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATDIVEKEMYTFFDKGNPPESLTLRPEGTAGCVRALVEHNLLRGATPRVWYMGPMFRYEKPQKGRYRQFHQFGVETFGVATPDIDAELIMLTARLWKRMGVDHMVQLELNTLGETDERTEYRNALVAFLNEHKDALDEDSQRRLTTNPLRILDSKIESTQKILENAPKLHDFLKEDSLSHFQQLQDYLTAAGIKFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKADQSVPAVGFAMGMERLLLLLEQVE---QAEIVRHCEAFLVAEPA-YQSKALVLAEQLRDQLEAANSNIRIKTG-SQSSMKSQMKKADQAGAVYAIILGEREWEAQQLAVKELATAEQSQVALAELVPFLIEKFTK 1HTT Chain:D ((3-410)) --NIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNG--DSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEHVTLELNSIG------------------------------------------------------------DEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTN----QGTVCAGGRYDGLVEQLGGRA---TPAVGFAMGLERLVLLVQAVNPEFKADPV--VDIYLVASGADTQSAAMALAERLRDEL----PGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEVANGTAVVKDLRSGEQTAVA--------------

General information: TITO was launched using: RESULT: Template: 1HTT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1591 -123239 -77.46 -363.54 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -77.46 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1HTT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HTT-query.scw PDB file : Tito_Scwrl_1HTT.pdb: