Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIENPIKRRPTRKIRVGSVYVGGDAPISVQSMTNTETCDVDATVAQIERCVDAGADIMRVSVPSMEAAEAFGAIRKRV-----SVPLVADIHFDHRIALA---VADYGADCLRINPGNIGS----DQKVREVVAAARHHGISMRIGVNAGSLEKDLQKKYG-------------EPTGQALLESALRHIDILDRLDFH--EFKVSVKASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLAAEP----EDEIKIGFDILKSLGLRSNGINFIACPSCSRQEFN-VIQVMQALEERLED----------IRTPMDVSVIGCKVNGPGEAKEADIGVVGA-APR---SLVYRNGEKSHLIDTNQLVDEIETMVRQRVQELEEAKSKEIIRSSS 4S39 Chain:A ((4-404)) ------MRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVNDEEAAKAVPEIKRRLLAEGAEVPLVGDFHFNGHLLLRKYPKMAEALD-FRINPGTLGRGRHKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDRNARRPEPKSAHEVVLEALVESAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGIVASAAALAPLLLEGIGDTIRVSLTPAPGEPRTKEVEVAQEILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSRRLKERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKTRFAP----------

General information: TITO was launched using: RESULT: Template: 4S39.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1845 299 0.16 0.84 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 0.16 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_4S39.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S39-query.scw PDB file : Tito_Scwrl_4S39.pdb: