Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKNAKIKILDLDLRGCLYLNNFSHSQRVALFFKIISRAGDGPFWYLMLAIVWGMQGITYSLQIIYLLLGGSVGTAIYKFLKHKTTRPRPYQVHQVIV----LGERPLDHFSFPSGHTLHAVMVTIVLGYIQPVLLAAMLPFMVLVALSRMVLGLHYPSDVIVGALIGAAVASLIIFVAPLFGIAL 1EOI Chain:B ((107-211)) --------------------------------------------------------------------------GDLATRSAKDHYMRIRPFAFYGVSTCNTTEQDKLSKNGSYPSGHTSIGWATALVLAEINPQRQNEILKRGYELGQSRVICGYHWQSDVDAARVVGSAVVATLHTN--------

General information: TITO was launched using: RESULT: Template: 1EOI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 442 -35165 -79.56 -348.16 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -79.56 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_1EOI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EOI-query.scw PDB file : Tito_Scwrl_1EOI.pdb: