Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIQGKHFVITGGGSGLGAATAEYLVKQGASVTLVDMNVEAGEQQAKHLGPKAGFVKLDVTDEATAEQFFKDVLVKHGHLHGLVNCAGIGPSAKVVGREGVHDLGLFAKTLNINVTGTFNMLRFAADAMSKNTVEEGEEDRGIIVNTASVAAFDGQIGQAAYSASKGAIVAMTLPIARELARHAIRIMTIAPGIMETPMLKGMPQNVQDALGQMVPYPSRLGKPEEFARLVAHIAENSYLNGEVIRLDGAIRMAAK 4PN3 Chain:B ((9-263)) MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDVASDTDGKAAIAAAIEAFSRIDVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFNMLRLAAEAMAKNEPGQGGE-RGVIINTASVAAFDGQIGQAAYSASKGGVAAMTLPVARELARHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLNGEVIRLDGALRMAAK

General information: TITO was launched using: RESULT: Template: 4PN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1422 -140692 -98.94 -551.73 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -98.94 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.607

(partial model without unconserved sides chains): PDB file : Tito_4PN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PN3-query.scw PDB file : Tito_Scwrl_4PN3.pdb: