Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCNKASSDLTDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKKIALTDLYFPQLGIHIEVDEGH-HFLRNSK---MEYSLNQIDEPLYSISQTESDAMREEDIISITGHKIFRVNVFKNQEGQPQNLENIHQQID----KIIEEIKTAKNKLIEASTFKEWNIETEYNPQTYIDLGRISLADNVVLKTTKDVCNCFGYSYKNYQRGGALHPYKKDTLIWFPRLYENKDWINTISPDGLTITEKSTDETITLKKLEEWKNGPQKRIVFARVKDNLSSRAMYRFMGLYEFQKADLKDGAVWKRVKCEVQTYSPKETKC 1CW0 Chain:A ((23-140)) ----------------------------IEKRLASLLTGQGLAFRVQDAS----LP-GRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLE--KIGKNVERDRRDISR-LQELGWRVLIVWE-----------CALRGREKLTDEALTERLEEWICGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CW0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 389 -19095 -49.09 -173.59 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -49.09 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_1CW0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CW0-query.scw PDB file : Tito_Scwrl_1CW0.pdb: