Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSICIFCGSSLGSNPIFQQIAQLTGEAIAKQGKTLVYGGGRSGLMGVVADSALQAGGQVIGVIPRALVDRELAHPGLTKLYVVENMHERKTKMADLSDGFIALPGGAGTLEEIFEQWIWAQLGIHQKPCAFLNVAGFYEDLLKMIQGTVDNGFSQARFVDKLIASDKIEDILQQFEQYQAPAPKWTNADVQP 3SBX Chain:H ((15-185)) --TVAVYCAAA-PTHPELLELAGAVGAAIAARGWTLVWGGGHVSAMGAVSSAARAHGGWTVGVIPKMLVHRELADHDADELVVTETMWERKQVMEDRANAFITLPGGVGTLDELLDVWTEGYLGMHDKSIVVLDPWGHFDGLRAWLSELADTGYVSRTAMERLIVVDNLDDALQ-------------------

General information: TITO was launched using: RESULT: Template: 3SBX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 883 -134037 -151.80 -783.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain H : 0.77 3D Compatibility (PKB) : -151.80 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3SBX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SBX-query.scw PDB file : Tito_Scwrl_3SBX.pdb: