Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MLMSNHL----IKHFQKGKRMQLATKSFLGLCLICSTAGAV--EPAATPATPPTAA-STQNAKPAPTSATVAKPATTTAPTTTQ-----------PLTAEQIAKNKQQ-QDAKIKAL-----------VQDQASRLKQLEHA-----------NLEAL---AQNQEL-QLKN-DNLSVQVQVLQSERSAQMFLYGAVTLAGGVLLGFFIASYVYTKRRRQW-------------------------------- 4D05 Chain:A ((1-258)) GQPPPIQLATNYRQDIDVTQYYVSEKLD-GIRAYWNGHQLISKQGNIFTAPTWFIASFPTTAMDGELWIARQQFETVSGIARTQDNQNEQWKQIKFMIFDLPKSTVSFEQRINKMQTLVTDTNSPYLQMIEQQKIPNTVALFDLLNKVVMGKGEGLMLHHQDALYQTKRSRDLMKLKKFEDAEATVIAYLPGKGKYEGLLGAILVKNEEGVTFKIGSGFSDEERSTPPPIGSLITYRFTGKTNNNIPRFASFVRIRVIY

General information: TITO was launched using: RESULT: Template: 4D05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 822 54520 66.33 313.33 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 66.33 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_4D05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D05-query.scw PDB file : Tito_Scwrl_4D05.pdb: