Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTALDQLKTL-TTVVADSSDLEAIRKFRPLDATTNPSLITAAAEQPESKELIEDAYYQAKEEGYKNDELIERTIDILTVKFGVEILKLIEGRVSTEVDTALSYDTEATIQKAHELCELYKGYGIDQSRILIKIASTWEGIQAAKVLEAE-GIACNLTLLFGLHQAQACADAKVTLISPFVGRILDWYKKAEGVDSYPIEKDPGVVSVKKIYTYYKQQNIPTQVMGASFRSIDQVLGLAGCDLLTISPSLLTQLEQDTRTVDAALDANKAKQAEAI--VRPAQDEQSFKDELNHDLMAFQLLQGGVDGFIKARDQLSLLLRQSFGIDAEIKS 3CQ0 Chain:B ((14-318)) ---SSLEQLKKAGTHVVADSGDFEAISKYEPQDSTTNPSLILAASKLEKYARFIDAAVEYGRKHGKTDHEKIENAMDKILVEFGTQILKVVPGRVSTEVDARLSFDKKATVKKALHIIKLYKDAGVPKERVLIKIASTWEGIQAARELEVKHGIHCNMTLLFSFTQAVACAEANVTLISPFVGRIMDFYKA------YTAETDPGVLSVKKIYSYYKRHGYATEVMAASFRNLDELKALAGIDNMTLPLNLLEQLYESTDPIENKLNSESAKE-EGVEKVSFINDEPHFRYVLNEDQMATEKLSDGIRKF-----------------------

General information: TITO was launched using: RESULT: Template: 3CQ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1687 4166 2.47 14.07 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 2.47 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_3CQ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CQ0-query.scw PDB file : Tito_Scwrl_3CQ0.pdb: