Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPSISLIREIDALLPQTQCGLCGHRDGCLPYAKSIAEGEEANKCVPGGQPVADALAQLLNRPLLPAETSVWPVQADGRPQRMKAIIREDECIGCTKCINAC---PVDAIIGSGKLMHTILTDLCTGCELCIPPCP-VDCIDLVEDTQALPSEQQRFAEQNDLRQRYYAHIQREEKRRIHRKGPVVRAEIDTQLFAQFSQALDDLPSIQLIEKPKEVIVSDAKTTIELAKIRTQIKKLEKQLSVREDAKKRLQLDELQQQLIQLQEV 1GTH Chain:C ((942-1015)) --------------------------------------------------------------------------------EQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTT--PYEPKR---------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GTH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 283 -27364 -96.69 -390.91 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -96.69 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1GTH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GTH-query.scw PDB file : Tito_Scwrl_1GTH.pdb: