Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------MSTQPMIEKLIEAHLDFLDEQFAQ--------------TQVIQQEFEQFYHWL-GSRQLQHLWTFEQV-------QQLIQKQILDTPASDFL---IEQIAEHIRFALIHPTNDTATVEDVIPVLTIDRIAQYVASKEEHRKKLIKTIVNNPAFSALLTQLIQQTMHDYLDESMTKRVPGVGRFMKMGKSVLETVTDSNLDDTINHYLQKNILKLSQMSKRVLNQHFDNDKLYHFQAN------------VWHKVKTS--PLSVLKNYIEV-QDLTRTVGLGHEIWDH----------IRQTDYLKQQVHDGI--------YTWYVRNQERNFDLLLRD----LNIDENLVKHELTELLVPV----LQQLVTTGHLRRRARVYLEKFYYSEKALEILNNKGA--------- 4K70 Chain:A ((2-483)) PGSMEALVRALEEADHAVATVVQSRILEFFMAAGRETPAGVRGLWARALRLACRAYVETGTCEAAVLAENLAGLALWRLRHDWDEGTAPLLELLGVVNGDDTTAALTEAGLRTSAEFGPDAMFRLVSEWCAAFDEALAGARSADDVLAAPRVVPPEQTARALVQPRFATLYDMDFVQDGLRYVAQHTNWALPLALAVRQMQNEGLKPLTRALFALTIADEFFHDRQ-------NPTLREQFAEAARA-----VDEAALVPVGEVNATPRTAVEVRVSAALAHGDAYVRELRPGTVARRLRTDQGVLALLDPGAQAVHVAAAADLDHTQVDATGVWEAVQASASPLQVVEALVTAGFTRRHCDLLERAVLDRAPRLTDAQRAVGCTAVVGGVVHRLLDDYGPGLDYVRAYTDVADTLEPLYGDVTAALGLPEKGVEHVVRHCMAPRPPTEHVGAARAALLREVAAAERRAGLAHSAAREALNTWLAFRAQSRWGL

General information: TITO was launched using: RESULT: Template: 4K70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1482 10223 6.90 31.65 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 6.90 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_4K70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K70-query.scw PDB file : Tito_Scwrl_4K70.pdb: