Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKTLPLTDIHRHLDGNIRIQTILELGQQYNLDLPAYDIESLRPHVQVMDNQPDLLSFLSKLDWGVKVLASL-DACKRIAFENMQDAAQQGLDYVELRFSPGYMGMAHQLPL-----------EGVVEAVIAGVKEGSQAYGVKANLIGIMSRTFGQEACEKELNALLAHKND--IKALDLAGDELGFPGNLFIDH---FKKARDAGWHITVHAGEAAGPESIWQAIEELGAERIGHGVKAVQDLKLLDYLAKHQIGIESCLTSNIQTNTVPSLAEHPLKTFLEHGVLATINTDDPAVEGIEIQHEYLTAAPLAGLSPEQIYTAQENGLKIAFLSEQEKDELRQKYQ 1A4L Chain:B ((7-332)) ------PKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELR-NIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRH--QPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFL-------------

General information: TITO was launched using: RESULT: Template: 1A4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1832 31443 17.16 101.76 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 17.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_1A4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4L-query.scw PDB file : Tito_Scwrl_1A4L.pdb: