Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIQNTILGKIVDRKHEDLAARLKQRNLQDVEELAKAATPVRGFANALQHKRPGVIAEIKKASPSKGIIRADFNPAEIAQQYEQAGAACLSVLTDVDFFQGADENIAIARNHCTLPALRKDFLVDPYNVVEARALHADCILLIVACLSDQQLEEMSKTAFEHQLDVLVEVHDEEELERALKLSEQCLLGVNNRNLKTFDVDLNTTIRLKKLLPASRLLITESGIATPDDVRMMQEHDIHSFLVGESFMKQPRPDQAFTALFGQPQTV 1JCM Chain:P ((1-258)) ----MQCVLAKIVADKAIWVEARKQQQPLASFQNEVQ--PSTRHFYDALQGARTAFILECKKASPSKGVIRDDFDPARIAAIYK-HYASAISVLTDEKYFQGSFNFLPIVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSNEEEQERAIALG-AKVVGINNRDLCDLSIDLNRTRELAPKLGHNVTVISESGINTYAQVRELSH-FANGFLIGSALMAHDDLHAAVRRVLLGENK-

General information: TITO was launched using: RESULT: Template: 1JCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1432 -172495 -120.46 -668.58 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain P : 0.79 3D Compatibility (PKB) : -120.46 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_1JCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JCM-query.scw PDB file : Tito_Scwrl_1JCM.pdb: