Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITANKGEWSEVYALFKLLSEGKLYAGDKDLNKIPNLIYPIISILRQESQDLLTYSPEPNHGVVHIQNGTSTFTISQSDFKGITELLLTEIKKKQPTASFSIPEVETFISQYNSKKIKAKSSAKSDIRIIIYDQKIGTTPELGFSIKSKLGKASTLLNASQATNFVYKVKNLTLSPQ-EIDDFNQLEFTVPIIQGRIHHLESLGGSVEFSHITNDVFNNNLILLDSLLPNIISEALYKYYTSPITSVKEIFEQVASANPIHYSLQHQHPFYEYKVKKLLCEIAIGMMPSTVWTGNNIDATGGYLVIKEDGDVICYHLYHRHEFEEYLYNNTR--FEAASKSRHN--------YGNLFIENGELYMTLNLQIRFK-- 2W0C Chain:L ((1-335)) MIVKKK------------LAAGEFAETFKNGNNITI-------IKAVGELVLRAYGADGGEGLRTIVRQGVSIKGMNYTSVMLHTEYAQEIEYWVGDLDYSFQEQTTKSRDVNSFQIPLRDG----VRELLPEDA----SRNRASIKSPVDIWIGGENMTALNGIVDGGRKFEAGQEFQINTFGSVNYWVSDEEIRVFKEYSARAKYAQNEGRTALEANNVPFFDIDVPPELDGVP--------------FSLKARVRHKSKGVDGLGDYTSISVKPAFYITEGDETTDTLIKYTSYGSTGSHSGYDFDDNTLDVMVTLSAGVHRVFPVETELDYDAVQEVQHDWYDESFTTFIEVYSDDPLLTVKGYAQILMERT

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1480 16337 11.04 50.73 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain L : 0.63 3D Compatibility (PKB) : 11.04 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.083

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: