Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MMKKEIVLTA-----AMMLFS---------MVASTTFVSATEVYPKEYNTEGTITFEAGDEGVTPPVDPENPDPNKPVDPS-DPPSPGTGGALSIDYGSKFKFG-------TQKISTADKTYYAAADVMNDGSRKPT------YVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQ---------AVSLVDPTYTPQTVNS-----ELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN 2YN5 Chain:A ((1-283)) TPPNAPVVTYSDIVNDLIIMQGTAEAKSQLIITDSEGNTYTLTVPDNGKWSMAIPYPSEGKFTITSVDAIGNRSDDVPLDIMKEVPVISLSPDSDSGTVGDN--ITRDKQPTFIIGNLESDVVVVQVDINGTVYNAEKNADGVWFFTPGTPLADGSYTISVIASDAAGNQKN-SLPITVTIDS--TLTVPEIALAAGEDNGASDSDNVTNHTQPKFTLQHIDADVTGVTVNVTHNGVTDIYQATQGADG-----WTFTPPAAWNDGNYTLSVTVVDRAGNSQQSASLAVTVDS----

General information: TITO was launched using: RESULT: Template: 2YN5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 985 50117 50.88 218.85 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 50.88 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_2YN5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YN5-query.scw PDB file : Tito_Scwrl_2YN5.pdb: