Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKGASPMKAVTWQGKQKMEIRNVDDPKI------LEPTDAIIRITATAICGSDLHLYHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMESQCDES--NPDQLYGGLFGFGKLNGNHW-GGQAEYLRVPYADSTSFVVPDNDLPDEKV---LFLSDILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKAANYNGVETYFLEDTAQAGTELYE-LAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGTISP---LQMASQAVKKFGTVQVTGVYM--------TPASSYPLQ----EFFMRDIAVKHGQAPIIHLMPKIYAMIAEGLFDPTQIIT-----HSLPLSEAAQAYEIFDKKEDKNIKVVLKP 2DPH Chain:B ((4-381)) ---------KSVVYHGTRDLRVETVPYPKLEHNNRKLEHA-VILKVVSTNICGSDQHIYR-GRFIVPKGHVLGHEITGEVVEKGSDVELMDIGDLVSVPFNVACGRCRNCKEARSDVCENNLVNPDADLGA-FGF-DLKG--WSGGQAEYVLVPYADYMLLKFGDKEQAMEKIKDLTLISDILPTGFHGCVSAGVKPGSHVYIAGAGPVGRCAAAGARLLGAACVIVGDQNPERL-KLLSDAGFETIDLRNSAPLRDQIDQILGKPEVDCGVDAVGFEA---------HGLGDEANTETPNGALNSLFDVVRAGGAIGIPGIYVGSDPDPVNKDAGSGRLHLDFGKMWTKSIRIMTGMAPVTNYNRHLTEAI---LWDQMPYLSKVMNIEVITLDQAPDGYAKFDK------------

General information: TITO was launched using: RESULT: Template: 2DPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2129 3969 1.86 11.50 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 1.86 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_2DPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DPH-query.scw PDB file : Tito_Scwrl_2DPH.pdb: