Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYLQDIDQQNMEGRKMKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENY-----IPSDMPAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALLAYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTD-GWGHIAADGSIANLEGLWYARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRSGKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELAGQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFLNEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYAAGGIVIKHKN-MRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLPLNVTGYGQLIGASIEAAQRFREFLDHL-TFTVKGKTIEVYPLNHPDFNMVNWVFKEQGCTDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEEWKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ 4RM1 Chain:C ((3-480)) -------------AMDSRFLPATAFIDPEGRNRNEVERLVQQVVDLILAKLTGAAERPPMPET---L-PGPITIP---EAAATEATLLQAIRDMV-DGSMNPA-NPGYIGHMDPMPATMAILGDLVAAAVNNNMLSLEMSPSFSRLETLLLRAIAGLFGLGEQAGGVLTSGGSLANLQALAVARNVAFDSVEPG-------------------------I------------------TGL--AQRPVIFASEAAHTSLQKAAMLLGLGTAAVIPVRATADSRMDPEDLRARIDQARGAGQHPFCVVATAGTTTTGNIDPLAEIGAIARE-----HGLWFHVDAAYGGALVFS-------------------------------ERHRWRLAGIEQADSITFNPQKWLYVAKTCAMVLFRDAGVLERAFRIPAPQMRA-D--GFINLGEIGVQGTRH-ADVVKLWLTLQHIGQ--QGYARLIDDGYRLAERVVEGVRQRPFLR-------LA--GEIDTNIVCFRGEPDW-LPAERWDDWNAALQALLLRE------GKIFLSLPVYR--------------------------GGRWLRAVLLNPYTTDAVI-----DAMFKQIDRFAGR---

General information: TITO was launched using: RESULT: Template: 4RM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2492 -17620 -7.07 -37.73 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -7.07 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4RM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RM1-query.scw PDB file : Tito_Scwrl_4RM1.pdb: