Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNLSKVCKGVRQLKKYGPFLA------IFFIALA-----FLMPHL-----------FTHKLILGADAPFHYNRFYDAAQQIKYGDF---HYFPSLYGFQQSGRI---------------VNAVYGPFFAYLQGLIVLLAGSWFHYQIL---SNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTSWGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYF--------FNQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGSYWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKNVPFISLIQFPFRFFVPF-----TLLLLLYLALSLNQWSEQKWFRL----LSIFGILIFSAQTIQNLYQHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDNKYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTDYEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA 1QNI Chain:A ((10-581)) ----AHVAPG--ELDEYYGFWSGGHQGEVRVLGVPSMRELMRIPVFNVDSATGWGITNESKEILGGDQQYLNGDCHHPHISMTDGRYDGKYLFINDKANTRVARIRLDIMKTDKITHIPNVQAIHGLRLQKVPKTNYVFCNAEFVIPQPNDGTDFSLDNSYTMFTAIDAETMDVAWQVIVDGNLDNTDADYTGKYATSTCYNSERAVDLAGTMRNDRDWVVVFNVERIAAAVKAGNFKTIGDSKVPVVDGRGESEFTRYIPVPKNPHGLNTSPDGK-----------YFIANGKL-SPTVSVIAIDKLDDLFEDKIELRDTIVAE-------------PELGLGPLHTTFDGRGNAYTTLFIDSQVCKWNIADAIKHYNG--DRVNYIRQKLDVQYQPGHNHASLTESRDADGKWLVVLSKFSKDRFLPVGPLHPENDQLIDISGEEMKLVHDGPTYAEPHDCILVRRDQIKTKK----IYERNDPYFASCRAQAEKDGV----TLESDNKVIRDGNKVRVYMTSVAPQYGMTDFKVK---EGD-EVTVYITNLDMVEDVTHGFCMVNHGVSMEISP-----QQTASVTFTAGKPGVYWYYCN----WFCHALHMEMVGRMLVEAA

General information: TITO was launched using: RESULT: Template: 1QNI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3167 -87464 -27.62 -170.83 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -27.62 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.140

(partial model without unconserved sides chains): PDB file : Tito_1QNI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QNI-query.scw PDB file : Tito_Scwrl_1QNI.pdb: