Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKENFKRSVSRMNAFRSFQVIKEP--------------VFHTAISDLPFTELAENEVLVKIAYSDVNYKDALAMSES-------------GQV----IRTYPMTPGIDLSGTVVQSNN--PRFSKEDLVLATGFG--------------------LGV-THPGGYSQYQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGVGSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFL-----------------P-ETTKA-LGKQQIDYVIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPIDQRLSLWQQMADLQIA--DEL-VVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK 3KRT Chain:A ((22-420)) ------IAALPLPESYRAITVHKDETEMFAGLETRDKDPRKSIHLDDVPVPELGPGEALVAVMASSVNYNSVHTSIFEPLSTFGFLERYGRVSDLAKRHDLPYHVIGSDLAGVVLRTGPGVNAWQAGDEVVAHCLSVELESSDGHNDTMLDPEQRIWGFETNFGGLAEIALVKSNQLMPKPDHLSWEEAAAPGLVNSTAYRQLVSRNGAGMKQGDNVLIWGASGGLGSYATQFALAGGANPICVVSSPQKA-EICRAMGAEAIIDRNAEGYRFWKDENTQDPKEWKRFGKRIRELTGGEDIDIVFEHPGRETFGASVFVTRKGGTITTCASTSGYMHEYDNRYLWMSLKRIIGSHFAN------YREAWEANRLIAKGRIHPTLSKVYSLEDTGQAAYDVHRNLHQGKVGVL-----

General information: TITO was launched using: RESULT: Template: 3KRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1742 -38391 -22.04 -118.86 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -22.04 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3KRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KRT-query.scw PDB file : Tito_Scwrl_3KRT.pdb: