Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEESMRIACYGVRPNEIDFFE-KLNIYHYDLSLYEELLTHDNIVSAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEAVLFKGLGAKVIGYDIHQTEAAKKV-LTFHSLEELLAKSDIVSIHVPHIPGENDQMINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQ--------QLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR 4ZGS Chain:F ((34-368)) ----TTRCICYSTTQYVKDFLAGPMQKVFTDTYFVEPPLDKDTAQLARGYDVAVLFVNDRADASVIKELAKAGVKLIALRCAGFDRVDLHACAEHGVRVVRVPTYSPESVAEHAVALIFALNRHLTDAYIRVRMGNYSL-SGLVGVEMRHKVVGVVGTGAIGQQAARILKGIGCKVFAYDIKPNPAVEAMGIPYVSLDELLAMSDIVTLHCPLLP-STRQLINKESIQKMKKGVMLINVSRGGLIDSAALFDALESGQIGALGLDVYENEGGLFFVDHTKF----DPSVRMQKWDRQFRTLLSYPQVLVTPHTAFLTEEALNNICTTTIQNIADYVLDRPLGNEV---------

General information: TITO was launched using: RESULT: Template: 4ZGS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1653 -24747 -14.97 -76.14 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain F : 0.78 3D Compatibility (PKB) : -14.97 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4ZGS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZGS-query.scw PDB file : Tito_Scwrl_4ZGS.pdb: