Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKV-------GGQWKFSERNLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYANKKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS 4LHF Chain:A ((7-87)) LMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTDPQSASAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGL-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 5864 32.94 82.58 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 32.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_4LHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LHF-query.scw PDB file : Tito_Scwrl_4LHF.pdb: