Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACVAGNSYGIGASVT-LPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFGPLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGR-TLAAEEDFVQAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFW-LNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE 4C5N Chain:D ((5-267)) ------KVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLE-TALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVC-------NPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKALDQDKS----YDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIE

General information: TITO was launched using: RESULT: Template: 4C5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1576 10680 6.78 42.21 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : 6.78 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4C5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C5N-query.scw PDB file : Tito_Scwrl_4C5N.pdb: