Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAISTVNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAENSEYIQIKTINDK 1S12 Chain:B ((1-91)) MIKVTVTN------SFFEVTGHAPD-----KTLCASVSLLTQHVANFLKAEKKA-K---IKKES-GYLKVKFEE-----LENCEVKVLAAMVRSLKELEQKFPSQIRVEVID--

General information: TITO was launched using: RESULT: Template: 1S12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 359 -55353 -154.19 -608.27 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -154.19 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_1S12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S12-query.scw PDB file : Tito_Scwrl_1S12.pdb: