Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDP-ISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 1MQ0 Chain:B ((7-136)) --ECVQQLLVCSQEAKQSAYCPYSHFPVGAALLTQEGRIFKGCNIENACYPLGICAERTAIQKAVSEGYKDFRAIAIASDMQDDFISPCGACRQVMREF-GTNWPVYMTKPDGTYIVMTVQELLPSSFGPEDL

General information: TITO was launched using: RESULT: Template: 1MQ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 654 -45415 -69.44 -352.05 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -69.44 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_1MQ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MQ0-query.scw PDB file : Tito_Scwrl_1MQ0.pdb: