Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------MTDKQKIDEFFWNKTTPEVARDL-LGMYLEHDTPEGRLAGYIVDAEAYLGP-EDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNI-----ITQPEGIPQGVMIRAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQE------LYGQSIADSSLRLVFEKKKTPKKIN-------------------------------ALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGSINIFR-- 2PJD Chain:A ((3-335)) AFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRAHTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLLPVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDAEKFWGEYSVDGLTVKTLPGVFSRDGLD-VGSQLLLSTLTPHTKGKVL-DVGCGAGVLSVAFARHSPKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQTSLDAAQTLIRGAVRHLNSGGELRIVANAF----LPYPDVLDETFGFHEVIAQTGRFKVYRAI

General information: TITO was launched using: RESULT: Template: 2PJD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1044 35214 33.73 162.27 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 33.73 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_2PJD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PJD-query.scw PDB file : Tito_Scwrl_2PJD.pdb: