Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVEWRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHVIHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVMEHFGADIGKLAVTPNNERDVLRLMELT-RRANAFVSMPIITMSMGNLGKISRLVGGTTGSVMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL 4GUI Chain:B ((7-254)) -VTVRDLVVGEGAPKIIVSLMGKTITDVKSEALAYREADFDILEWRVDHFANVTTAESVLEAAGAIREIITDKPLLFTFRSAKEGGEQALTTGQYIDLNRAAVDSGLVDMIDLELFTGDDEVKATVGYAHQHNVAVIMSNHDFHKTPAAEEIVQRLRKMQELGADIPKIAVMPQTKADVLTLLTATVEMQERYADRPIITMSMSKTGVISRLAGEVFGSAATFGAVK--KASAPGQISVADLRTVLTILHQ--

General information: TITO was launched using: RESULT: Template: 4GUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1374 -127048 -92.47 -514.36 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -92.47 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_4GUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GUI-query.scw PDB file : Tito_Scwrl_4GUI.pdb: