Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTHNDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKFYISSVPAHH-K---Y-PNVKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTS--NYSFIWAMCGENITYTDMDMVQMDQLK 1SFN Chain:B ((2069-2237)) -----------------------------------------------------------------------------QRFAFVLSGEVDVAVGGETRTLREYDYVYLPAGEKHMLT-AK---TDARVSVFEKPYQTVEGVQAPGVYWGNERENPGYPFE---GDDHLIARKLLP-D-EPAFDFMVSTMSFAPGA-S-LPYAEVHY-MEHGL-L-MLE---GEGLYKLE--ENY--YPVTAGDIIWMGAHCPQWYGALGRNWSKYLLY--------------------

General information: TITO was launched using: RESULT: Template: 1SFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 703 -19742 -28.08 -121.86 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -28.08 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1SFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SFN-query.scw PDB file : Tito_Scwrl_1SFN.pdb: