Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPELAKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIRGDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK 1BHN Chain:E ((5-136)) -ERTFIAIKPDGVQRGLIGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIALWFHP------------

General information: TITO was launched using: RESULT: Template: 1BHN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 616 -81186 -131.79 -615.04 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -131.79 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_1BHN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BHN-query.scw PDB file : Tito_Scwrl_1BHN.pdb: