Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHEPADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPKER-RGLS-VKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII 4EGG Chain:C ((17-186)) ----FDQTLINERLRAKVICFALNHT------NPVATM---------MRKVLIDALFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKIDN--

General information: TITO was launched using: RESULT: Template: 4EGG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 840 -70532 -83.97 -419.83 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -83.97 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4EGG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGG-query.scw PDB file : Tito_Scwrl_4EGG.pdb: