Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKEIKRRNRMKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIGVLQFV-SHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGNDLVVGIATPAAQGLASA--TKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQVELIKALTPNVKTIGALYSSSE--DNSKTQVEEFKAYAEKAGLTVETFA--VP--STNEIASTVTVMT---SKVDAIWVPIDNTIASGFPTVVSSNQ--SSKKPIYPSATAMVE---VGGLASVVIDQHDLGVATGKMIVQVLKGAKPADTPVNVFSIGKSVINKKIAQELGITIPESVLKEAGQVIE 1RZR Chain:C ((57-314)) ----------------------------------------------SKKTTTVGVIIPDISNIFYAELARGIEDIATMYKY-----NIILSNSDQNQDKELHLLNNMLGKQVDGIIFMSGNVTEEHVEELKKSPVPVVLAASIESTN----------QIPSVTIDYEQAAFDAVQSLIDS--GHKNIAFVSGTLEEPINHAKKVKGYKRALTESGLPVRDSYIVEGDYTYDSGIEAVEKLLEEDEKPTAIFVGTDEMALGVIHGAQDRGLNVPNDLEIIGFDNTRLSTMVRPQLTSVVQPMYDIGAVAMRLLTKYMNKETV----------------------------------------

General information: TITO was launched using: RESULT: Template: 1RZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1278 726 0.57 3.01 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 0.57 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_1RZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RZR-query.scw PDB file : Tito_Scwrl_1RZR.pdb: